Gregory House, M.D., is a maverick medical genius, who heads a team of young diagnosticians at the fictional Princeton-Plainsboro Teaching Hospital in New Jersey. Most episodes start with a cold open somewhere outside the hospital, showing the events leading to the onset of symptoms for that week's main patient. The episode follows the team in their attempts to diagnose and treat the patient's illness.
House's nationally-renowned department typically only sees patients who have failed to receive a correct diagnosis at other hospitals, so the cases tend to be exceptionally complex and subtle. Furthermore, House tends to resist accepting cases that he does not find interesting. The medical cases featured are often rare but realistic, and described by Andrew Holtz, the author of The Medical Science of House, M.D., as "a conglomeration of all the worst things that can happen to people from all over the world, crammed into one little community
# 葛瑞格利·豪斯(Dr. Gregory House) (休·勞瑞) (Hugh Laurie)– 詳噌t學部主任,專攻傳染病及腎臟疾病。畢業於約翰·霍普金斯大學和密西根州大學, Dr House似乎缺少臨床詳嗟呐d趣, 喜歡盡最大可能避免與病人直接接觸。他經常說:「每個人都說謊!我們不接觸他們他們就沒法說謊!」 由於一個位於他右腿的血管瘤, Dr House失去了重要的肌肉部分,他必須依賴一根拐杖來行走. 另外,House 經常需要忍受肌肉疼痛, 他需要依賴止痛藥維可丁來緩解疼痛,最近甚至用起嗎啡。儘管他的舉動有些反對社會或者厭惡人類,Dr. House 不受約束的非傳統思考方式和自覺卻使他得到了同事的尊敬和包容。
# 麗莎·卡蒂(Dr. Lisa Cuddy) (麗莎·愛登斯坦)(Lisa Edelstein) – 醫院院長、內科部主任,專攻內分泌。 雖然平時看上去像個老是給Dr. House惹麻煩的傢伙,Dr. Cuddy事實上經常幫助 House 完成他那些怪異的醫學請求。聰明的Dr. Cuddy在故事中是第一個成為霍普金斯大學醫學主任的女醫生。另外,Dr. Cuddy在劇中是少數能夠趕上 House 說話速度的人物。雖然此劇的編劇嘗試讓 Cuddy 和 House 之間存在一種性緊張的關係,但為了給觀眾留下懸念,至今此事仍然沒有澄清。Cuddy 和 House 雖然同一時期在密西根大學就讀,但觀眾不知道他們當時到底認不認識。為了有個小孩,她正在秘密接受受孕治療。